ACC Laboratory Equipment  
     
 
  Common Equipment
  • Milliq water system
  • Typhoon **
  • Micro/standard top-loading balance
  • Perkin Elmer g Counter
  • BioRad Gel Doc
  • Image Eraser (Autoradiography)
  • Still
  • ABI Prism - Real-time PCR **
  • ThermoHybaid PCR
  • BioRad Spectrophotometer 3000
  • Thermo Hybaid Thermocyclers
  • LN2 Storage
  • Autoclave **
 
  Pharmacology Lab
  • MDS Sciex 3200 Q-TRAP triple quad mass spec-ACC 263
  • Turbo IonSpray Source
  • Agilent 1200 Series Binary Pump HPLC system
  • HTC Pal LEAP Autosampler
  • Parker Balston Source 5000 N2 generator
  • Xenogen Imager **
  • Savant Speed Vac Systems (2)
  • Sonic Dismembrator
  • Anesthesia Machine
  • Mouse Metabolic Cages (4)
  • Microcentrifuge
  • Shaking water bath
  • Analytical Scales (2)
  • pH Meter
  • Vial crusher
 
  Molecular Pathology
  • Ventana Medical Systems Discovery XT automated stainer
  • Carl Zeiss Axioplan 2 imaging scope
  • AxioCam HRc Carl Zeiss camera
  • Axio Vision 4.3 software from Carl Zeiss
 
  Tumor Immunology
  • Dako Flow Cytometer
  • Nexelcom Cell Counter
  • High-speed centrifuge
  • Virtis Lyophilizer
  • Leica Cryostat
  • Sonicator
  • Perkin-Elmer g Counter
 
 
 
 
ACC Laboratory Skills
 
   Cancer Genetics - Molecular
  • PCR/Real Time PCR
  • DNA analysis/sequencing
  • Gene Chip Arrays
  • Restriction Digests
  • Cloning/subcloning
  • PCR based generation of mutant constructs
  • Western Blots/Northern Blots/Southern Blots
  • Immunoprecipitation
  • Licensed to handle radioactive materials
 
   Cancer Genetics - Protein-to-Protein
  • Immunoprecipitation
  • GST - pulldown
  • Biacore
  • Yeast two-hybrid screening
  • In vitro transcription/translation for protein expression/labeling
  • Tissue Culture Skills:
  • Transient and stable cell transfection
  • Creating clonal isolates
  • IHC
  • MTT
  • Scratch Tests (Cellular Assays)
 
   Molecular Pathology
  • PCR/RT-PCR
  • RNA isolation
  • cDNA synthesis
  • IHC/IF
  • Western Blots/Dot Blots
  • Necropsy/Histology
  • Image Analysis
  • Lab animal care
  • ISH/FISH - set up, but never done
 
   Investigational Therapeutics
  • Tissue culture - primary and established mammalian lines
  • Bacterial culture
  • Migration/Invasion Assays
  • Flow cytometry
  • Growth inhibition assays
  • Anchorage - independent assays
  • ELISAs - commercial and "home-grown" development
  • Cryosectioning
  • IHC/IFC
  • PBMC/BMMC isolation
  • Cryopreservation
  • Western Blots
  • RT-PCR/qRT-PCR
  • LBT/MLR assays
 
 
 
  Medical Oncology
  • Thermo-Spectronic Biomate 3 spectrophotometer
  • Labsystems Multiskan MCC340 plate reader (absorbance)
  • Sratagene 2400 Stratalinker
  • Fisher mini- and maxi-gel boxes
  • Invitrogen Powerease 500 power supply
  • Invitrogen Vertical Gel system
 
  Cancer Genetics
  • Inverted fluorescent microscope
  • Gradient PCR machine
  • Life Technologies Model 4001P/BioRad Power Pak 1000
  • 3' Level II BioSafety hood dedicated to PCR
 
  Investigational Therapeutics
  • Refrigerated high-speed centrifuge, swing-out rotor/microfuge rotor
  • LN2 storage system
  • Electronic scale, Analytical balance
  • BioTekT Plate Reader - luminescence, fluorescence, absorbance
  • Inverted phase microscope
  • Binocular light microscope
  • Electroporater
  • Stratagene Mx3000P real-time PCR
  • NovexT Gel apparatus
  • Tissue "Tearor"T
  • Life Technologies Model 4001P - programmable power supply
  • Dry heat blocks (2)
  • Rocker platform
  • Microfuge (2)
  • pH Meter
 
   Musculoskeletal Lab
  • Surgery table/anesthesia machine
  • Xenogen imaging system **
  • Polytron
  • Microfuge
  • Refrigerated tabletop centrifuge
  • Rocker platform
  • UV-Vis spectrophotometer (temporary)
  • Inverted phase microscope
  • pH Meter
  • LN2 storage system
  • Sonicating water bath
 
 
 
 
 
 
   Cancer Genetics - Interactions
  • Electrophoretic Mobility Shift Analysis
  • South-Western Blot Analysis
  • Yeast One-hybrid screening
  • Luciferase Promoter Assays
  • Protein Expression and Isolation from Bacterial cultures
  • Inducible & constitutive gene expression in mammalian systems
 
   Pharmacology Lab
  • Mammalian tissue culture
  • Proliferation assays
  • Death/apoptosis assays
  • Immunocytochemistry/IHC
  • Light/flourescent microscopy
  • Flow cytometry
  • Western blotting
  • Animal handling, surgery, anesthesia
  • Plasma and tissue collection
  • HPLC/mass spectrometry
  • Biological sample extraction/ preparation pharmacokinetic modeling
  • Biochemistry gods
 
   Tumor Immunology
  • Immunostaining
  • Multi-color Flow Cytometry
  • Basic immunological assays
  • Flow cytometry - assays; instrument maintenance/troubleshooting
  • Tissue Culture
  • ELISA
  • PCR/Cloning
  • IHC/IFA
  • Protein Purification
  • Antibody/Protein labeling
  • Mouse wrangling - iv injection, grooming for tumor injections, surgical
 
   Musculoskeletal Lab
  • Xenogen imaging in vivo/in vitro **
  • Tissue culture
  • Bacterial culture
  • ELISA
  • Animal surgery, anesthesia
  • IHC
  • Western Blots
  • Xenograft models
  • Cryosectioning
  • Licensed to handle radioactive materials
  • Plasma and tissue collection
  • PBMC isolation
  • Light/flourescent microscopy
  • Necropsy
  • Animal handling (IV, IP, SC, tumor injections)
 
 
  ** included/proposed to be included in cost center (21 account)  
     
 
 
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